Experimental Hazara Virus infection in mice

Reproduction of Hazara virus in intracerebrally (ic) inoculated newborn white mice was studied by virological, serological and histological methods, electron microscopy and the direct fluorescent antibody technique. In the course of infection the virus was detectable in the blood, brain and some viscera, the largest amounts of virus occurring in the brain and liver. Pathomorphological lesions were localized in the brain and consisted of foci of neuronal destruction and marked oedema of the tissue. Electron microscopy of ultrathin sections of tissues from various parts of brains of infected newborn white mice killed at the height of infection revealed virus particles in perikaryon of the brain cortex neurons; accumulations of particles were found in vesicles of the Golgi complex and the endoplasmic reticulum.