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Updated: Jul 17, 2026

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
High-performance liquid chromatography with nuclear magnetic resonance detection applied to organosilicon polymers.
Vratislav Blechta1, Milan Kurfürst, Jan Sýkora
1Institute of Chemical Process Fundamentals, Academy of Sciences, Rozvojová 135, 16502 Prague, Czech Republic.
Abstract:
LC-NMR utilizing (1)H and (29)Si NMR spectroscopy is ideally suited for the analysis of silicones. It is shown that reversed phase gradient LC-NMR surpasses standard gel permeation chromatography (GPC) and diffusion ordered spectroscopy (DOSY) in the analysis of model hydride terminated polydimethylsiloxane. (1)H and (29)Si NMR in the stopped-flow arrangement leads to full identification of the components. Concentration gradient introduces a dependence of the (29)Si shifts on solvent composition, this dependence can be substantially reduced by a proposed method of referencing. It is shown that the ADEQUATE version of powerful but insensitive 2D INADEQUATE experiment can be used for complete line assignment.
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