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Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
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Detection of DNA with Catalytic Beacons Based on Peroxidase-oxidase Oscillating Reaction

Jinhong Zeng1, Qiong Liu, Huibi Xu

  • 1Chemistry Department of Huazhong University of Science and Technology, Wuhan Hubei 430074, China (e-mail: Jintao032961@163.com).

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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