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Related Experiment Videos

Proportional microvolume capacitive liquid level sensor array.

D Seliskar1, R Waterbury, R Kearney

  • 1Department of Biomedical Engineering, McGill University, Montreal, Canada.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
PubMed
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A novel sensor array enables precise, automated liquid level control in microplates. This non-contact capacitance system offers accurate measurements for biological and chemical research applications.

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Sensor Technology

Background:

  • Automated liquid handling is crucial for high-throughput screening and laboratory efficiency.
  • Accurate real-time monitoring of liquid levels in microplates is challenging, especially with varying liquid properties.

Purpose of the Study:

  • To develop and validate a non-contact capacitance-based sensor array for precise, closed-loop liquid level control in multiwell microplates.
  • To model fluid-level dependency and demonstrate the sensor's ability to compensate for differences in liquid conductivity and permittivity.

Main Methods:

  • Development of a non-contact capacitance-based fluid sensor model.
  • Extension of the electrical model to describe fluid-level dependency.
  • Design and fabrication of a prototype 3x3 sensor array.

Related Experiment Videos

  • Testing with NaCl and ethanol solutions to cover a range of conductivity and permittivity.

    • Main Results:

    • A linear relationship (R^2 > 0.92) was observed between calibrated sensor output and liquid volume for various solutions.
    • The sensor array demonstrated high accuracy, with an average measurement error of 1.3% (2.0 μl).
    • The system showed robustness, with a standard deviation of 6.0% (9.0 μl) in measurements.

    Conclusions:

    • The developed sensor array is feasible for accurate, automated liquid level monitoring in microplates.
    • The technology can be adapted for various M×N microplate formats and diverse research applications.
    • This advancement supports improved efficiency and reliability in automated laboratory workflows.