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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
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Study of Interaction Force between Antigen and Antibody using Flow Chamber Method.

Bo Gao1, Gang Jin

  • 1Institute of Mechanics, Chinese Academy of Sciences, # 15, Bei-si-huan West Rd., Beijing 100080, P.R.China; Graduate School of Chinese Academy of Sciences, # 19, Yu-quan Rd, Shi-jing- shan District, Beijing 100039, P.R. China.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
PubMed
Summary

This study quantifies antigen-antibody interaction forces using a flow chamber. Higher antibody concentration strengthens binding, and models were developed to estimate bond strength.

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Area of Science:

  • Biophysics
  • Immunology
  • Surface Science

Background:

  • Understanding antigen-antibody interactions is crucial for diagnostics and therapeutics.
  • Quantifying binding forces provides insights into molecular recognition mechanisms.

Purpose of the Study:

  • To investigate the interaction forces between human IgG and goat anti-human IgG using a parallel plate flow chamber.
  • To determine the relationship between ligand surface concentration and binding strength.
  • To develop and analyze models for estimating antigen-antibody bond strength.

Main Methods:

  • Utilized a parallel plate flow chamber for controlled shear flow experiments.
  • Immobilized human IgG (ligand) on a chip surface and goat anti-human IgG (receptor) on microspheres.
  • Measured the critical shear rate required to detach microspheres from the chip surface.

Main Results:

  • Demonstrated that microsphere-chip binding is mediated by bio-specific antigen-antibody interactions.
  • Found that the critical shear rate for microsphere detachment increases with higher ligand surface concentration.
  • Proposed and analyzed two models for estimating antigen-antibody bond strength, incorporating bond positions.

Conclusions:

  • The binding force is directly related to the bio-specific interaction between antibodies and antigens.
  • Increased surface concentration of antibodies enhances the overall binding strength.
  • Developed models provide a framework for quantifying antigen-antibody bond strength in complex systems.