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Related Experiment Video

Updated: Jul 17, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Silica nanoparticle tags for capacitive affinity sensors.

Abhishek Ramkumar1, Rakesh Lal

  • 1Bachelors in Technology in the Department of Electrical Engineering, Indian Institute of Technology, Bombay, Mumbai 400076, India, pursuing a Ph.D in Biomedical Engineering at Cornell University. ar339@cornell.edu.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
PubMed
Summary

This study introduces an indirect capacitive affinity sensor using silica nanoparticle tags to boost sensitivity for small analyte detection in immunoassays. This method significantly improves signal detection compared to direct sensing approaches.

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Area of Science:

  • Biosensor technology
  • Nanoparticle applications
  • Immunoassay development

Background:

  • Capacitive affinity sensors offer potential for disposable biosensors.
  • Direct sensing of small analytes with capacitive sensors suffers from low sensitivity.
  • Immunoassays require high sensitivity for accurate analyte detection.

Purpose of the Study:

  • To develop an indirect capacitive affinity sensor with enhanced sensitivity.
  • To utilize silica nanoparticle tags for signal amplification in sandwich immunoassays.
  • To demonstrate the sensor's efficacy using a human IgG-anti-human IgG model.

Main Methods:

  • Synthesized silica nanoparticles via controlled hydrolysis of TEOS.
  • Immobilized anti-human IgG antibodies onto silica nanoparticles.

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Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

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Last Updated: Jul 17, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

  • Constructed a capacitive immunosensor with a gold electrode on oxidized silicon, functionalized with human IgG.
  • Performed immunocomplexation and measured capacitance changes.

    • Main Results:

    • The indirect capacitive affinity sensor demonstrated enhanced sensitivity.
    • Silica nanoparticle tags significantly amplified the signal for sandwich immunoassays.
    • A notable decrease in capacitance was observed after nanoparticle-mediated immunocomplexation.
    • The sensor effectively detected the human IgG-anti-human IgG complex.

    Conclusions:

    • Indirect capacitive affinity sensors with silica nanoparticle tags are effective for improving sensitivity in immunoassays.
    • This approach overcomes the limitations of direct capacitive sensing for small analytes.
    • The developed sensor shows promise for sensitive and disposable biosensing applications.