Highly effective in vivo gene transfection by blocked star vector
1Department of Bioengineering, Advanced Medical Engineering Center, National Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
Abstract:
Nano-structured four branched, blocked star vector (BSV) was molecular designed by iniferter-based photo-living radical copolymerization method of N,N-dimethylaminopropylacrylamide and then N,N-dimethylacrylamide from four dithiocarbamate-derivatized benzene for a novel gene derivery non-viral vector. The conjugated pDNA complexes were satisfactorily stable in the condition of the electrophoresis. Under the charge ratio of 5/1 (vector/pDNA), complexes of BSV and pDNA (BSV-pDNA) demonstrated satisfied transfection efficiency into COS-1 cells with a little cytotoxicity. Mice injected with BSV-pDNA polyplexes showed a higher level of gene expression in either liver, kidney or spleen using the optimal charge ratio as determined in in vitro. These results suggest the potential use of BSV as a non-viral vector in clinic.
