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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
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Published on: February 16, 2018

Detection of Vesicular Stomatitis Virus using a Capacitive Immunosensor.

Darrin Hanna1, Brooks Gross, Elizabeth Lempicki

  • 1School of Engineering and Computer Science, Oakland University, Rochester, Michigan, USA.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
PubMed
Summary

A new capacitive immunosensor detects Vesicular Stomatitis Virus (VSV) with high sensitivity. This novel technology uses antibodies to detect VSV antigens, offering a promising tool for viral detection.

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Area of Science:

  • Biomedical Engineering
  • Immunosensor Technology
  • Virology

Background:

  • Vesicular Stomatitis Virus (VSV) poses a significant threat to livestock.
  • Accurate and rapid detection methods for VSV are crucial for disease control.
  • Existing detection methods can be time-consuming and require specialized equipment.

Purpose of the Study:

  • To develop and validate a novel capacitive immunosensor for the sensitive detection of Vesicular Stomatitis Virus.
  • To demonstrate the feasibility of using antibody-antigen interactions on a capacitive sensor for viral detection.
  • To establish the detection limit of the prototype immunosensor for VSV.

Main Methods:

  • Fabrication of a capacitive immunosensor using standard etching and metal plating techniques.
  • Immobilization of specific antibodies onto the sensor surface.
  • Detection of VSV antigens by measuring voltage changes due to capacitance alterations upon antigen binding.
  • Utilizing changes in capacitance as a direct measure of antigen presence.

Main Results:

  • The developed immunosensor successfully detected VSV.
  • Specific antigen binding to immobilized antibodies resulted in measurable capacitance changes.
  • The prototype device achieved a detection limit as low as 2 pg/ml for VSV.
  • The sensor demonstrated ruggedness, indicating potential for practical applications.

Conclusions:

  • A novel capacitive immunosensor provides a sensitive and specific method for VSV detection.
  • This technology offers a rapid and potentially field-deployable alternative to existing diagnostic tools.
  • The low detection limit highlights the potential of this immunosensor for early VSV outbreak identification.