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Related Experiment Videos

BAEC adhesion analysis using Thickness Shear Mode sensor.

S Hong1, E Ergezen, K Barbee

  • 1School of Biomedical Engineering, Science and Health Systems, Drexel University 3141 Chestnut St, Philadelphia, PA 19104.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
PubMed
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This study shows how a Thickness Shear Mode (TSM) sensor can monitor living cell adhesion in real-time. The sensor accurately detects different stages of cell attachment and behavior on surfaces.

Area of Science:

  • Biophysics
  • Cell Biology
  • Biosensing Technology

Background:

  • Cell adhesion is crucial for biological processes.
  • Real-time monitoring of cell attachment is challenging.
  • Thickness Shear Mode (TSM) sensors offer nano-scale penetration for sensitive detection.

Purpose of the Study:

  • To characterize the initial cell adhesion process using a TSM sensor.
  • To correlate TSM sensor responses with physical cell adhesion events.
  • To investigate cell adhesion under varied environmental conditions.

Main Methods:

  • Utilized a TSM sensor with nano-scale shear wave penetration depth.
  • Monitored impedance magnitude changes of bovine aortic endothelial cells (BAECs) during adhesion.
  • Validated sensor readings by rinsing surfaces and counting attached cells.

Related Experiment Videos

  • Investigated adhesion by modifying sensor coatings (gelatin) and blocking integrin binding (RGD peptides).

    • Main Results:

    • TSM sensor multi-harmonic responses characterized initial cell adhesion.
    • Sensor impedance changes correlated with cell sedimentation and attachment.
    • Distinct phases of cell adhesion were identified through sensor signal analysis (delay time, slope, maximum impedance change).
    • Adhesion behavior varied with gelatin coating and RGD peptide presence.

    Conclusions:

    • TSM sensors can effectively detect and differentiate phases of living cell adhesion.
    • This technology provides a label-free, real-time method for studying cell-surface interactions.
    • TSM sensing offers a valuable tool for biophysical and cell biology research.