Related Experiment Video

Updated: Jul 17, 2026

Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
09:05

Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells

Published on: July 22, 2015

Cell Lysis in SWLA-2 Hybridomas due to 1 kHz AC Electric Fields

Barbara Oakley1, Darrin Hanna, Sachin Kandlikar

  • 1Sch. of Eng. & Comput. Sci., Oakland Univ., Rochester, MI.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
PubMed

Abstract:

This paper describes results involving the percentage cell lysis of SWLA-2 murine hybridomas produced by AC electric field pulses at 1 kHz with pulse widths ranging from 1 ms to 1 second. Cells that had been exposed to the electric fields were cultured and replicate samples were examined at 48 hours to determine the number of viable cells.

More Related Videos

Cell Electrofusion Visualized with Fluorescence Microscopy
05:02

Cell Electrofusion Visualized with Fluorescence Microscopy

Published on: July 1, 2010

Electroporation of Functional Bacterial Effectors into Mammalian Cells
08:39

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Published on: January 19, 2015

Related Experiment Videos

Last Updated: Jul 17, 2026

Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
09:05

Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells

Published on: July 22, 2015

Cell Electrofusion Visualized with Fluorescence Microscopy
05:02

Cell Electrofusion Visualized with Fluorescence Microscopy

Published on: July 1, 2010

Electroporation of Functional Bacterial Effectors into Mammalian Cells
08:39

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Published on: January 19, 2015

Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

Articles linked to this work by shared authors, journal, and citation graph.

Accelerating the development of bioelectronic medicine: overview and impact of the NIH Stimulating Peripheral Activity to Relieve Conditions (SPARC) initiative.

Bioelectronic medicine·2026

Framework for advancing rigorous research.

eLife·2020

Learning how we learn: in an unlikely confluence, bioengineers are finding fertile ground in research and teaching about learning.

IEEE pulse·2015

Reducing hospital ICU noise: a behavior-based approach.

Journal of healthcare engineering·2014

Do higher dispositions for empathy predispose males toward careers in nursing? A descriptive correlational design.

Nursing forum·2014

Empathy as a determining factor for nursing career selection.

The Journal of nursing education·2013

Highly Articulated Robotic Probe for Minimally Invasive Surgery.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference·2010

Embedded assessment algorithms within home-based cognitive computer game exercises for elders.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference·2008

Digital signal processing of the wideband external pulse recorded during cuff deflation: a new way to measure blood pressure.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference·2008

Immune cells detection of the in vivo rejecting heart in USPIO-enhanced magnetic resonance imaging.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference·2008

wHospital: a web-based application with digital signature for drugs dispensing management.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference·2007

Wearable health systems: from smart technologies to real applications.

Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference·2007

Conditioned Media-Derived Tumor Extracellular Vesicles: Bridging Molecular Insights and Therapeutic Applications in Oncology.

Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology·2026

The Silent Intruder: A Case Report of an Incidentally Discovered Solitary Fibrous Tumor of the Lung.

Cureus·2026

Development and progression of hepatic steatosis during long-term glucocorticoid therapy in autoimmune hepatitis: multimodal evidence from biopsy, CAP, and ultrasound.

Frontiers in medicine·2026

An oral fentanyl self-administration model reveals dissociable escalation and relapse phenotypes in outbred vs inbred mice.

bioRxiv : the preprint server for biology·2026

Sex-dependent chronic neurological dysfunction following isoflurane anesthesia and surgery is associated with circulating extracellular vesicle-mediated neuroinflammatory signaling.

bioRxiv : the preprint server for biology·2026

Actively cycling cells in uninjured connective tissue are not a prerequisite for appendage regeneration.

bioRxiv : the preprint server for biology·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us