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CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV
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Published on: October 1, 2007

Clinical laboratories on a chip for human immunodeficiency virus assay

Wen-Peng Guo1, Xue-Mei Ma, Yi Zeng

  • 1Coll. of Life Sci. & Bioeng., Beijing Univ. of Technol.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.