Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimized nursing strategies for skin graft outcomes: a narrative review of current evidence.

Frontiers in surgery·2026
Same author

A Sparse Super-Resolution Imaging Approach for Array Scanning Radar in High-Resolution Ground Mapping.

Sensors (Basel, Switzerland)·2026
Same author

The influence of adolescents' perceived task value in sport on exercise adherence: the chain mediating roles of general self-efficacy and action planning.

Frontiers in psychology·2026
Same author

Feasibility of Ultra-Dilute Iodine Contrast Agent in Dual-Layer Spectral CT for Pulmonary Artery Imaging

Current medical imaging·2026
Same author

Effects of exercise on executive function in cognitively healthy older adults: a systematic review and three-level meta-analysis.

BMC public health·2026
Same author

Risk factors for impaired surgical wound healing after spinal tuberculosis surgery: a retrospective comparative trial.

Frontiers in surgery·2026

Related Experiment Video

Updated: Jul 17, 2026

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
09:47

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Published on: October 18, 2015

A 3-d microelectrodes array microchip applied to cell fusion.

Zhao Zhiqiang1, Zheng Xiaolin, Xiong Jiangong

  • 1Key Lab of Biomechanics and Tissue Engineering under the State Education Ministry, Bioenginering College, Chongqing University, 400044, China.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|February 7, 2007
PubMed
Summary

A novel 3-D microelectrode array chip enables high-throughput cell-to-cell fusion using lower voltages. This dielectrophoresis-enhanced electrofusion technique successfully fused human hepatocarcinoma cells.

More Related Videos

Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells
12:30

Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells

Published on: March 16, 2022

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip
08:35

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

Published on: December 29, 2015

Related Experiment Videos

Last Updated: Jul 17, 2026

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
09:47

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Published on: October 18, 2015

Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells
12:30

Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells

Published on: March 16, 2022

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip
08:35

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

Published on: December 29, 2015

Area of Science:

  • Biotechnology
  • Bioengineering
  • Cell Biology

Background:

  • Conventional cell fusion methods often require high voltages and lack efficiency for large-scale operations.
  • Developing efficient and scalable cell fusion techniques is crucial for various biological applications, including drug discovery and regenerative medicine.

Purpose of the Study:

  • To develop a novel 3-D microelectrode array microchip for simultaneous, high-throughput cell-to-cell fusion.
  • To investigate the efficacy of using lower alternating and pulse voltages for cell fusion compared to traditional methods.

Main Methods:

  • Fabrication of a 16 (4x4) microelectrode array on a silicon wafer.
  • Utilizing dielectrophoresis (DEP) force for cell alignment and pair formation.
  • Applying alternating and pulse voltages for cell fusion induction.
  • Testing the system with human hepatocarcinoma SMMC-7721 cells using 8µm carbon-fiber microelectrodes.

Main Results:

  • The microchip system successfully aligned and paired SMMC-7721 cells using DEP.
  • Cell fusion was effectively induced by applying electric pulses between the microelectrodes.
  • The technique demonstrated successful fusion of human hepatocarcinoma cells with significantly lower voltages.

Conclusions:

  • The developed 3-D microelectrode array microchip offers a promising platform for efficient, large-scale, low-voltage cell fusion.
  • This technology has potential applications in cell-based assays, drug screening, and synthetic biology.