Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bacterial Gastroenteritis

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effect of Short-Term Grape Powder Supplementation in Patients with Crohn's Disease: A Pilot Study.

Nutrients·2026

Same author

Infection by <i>Strongylus edentatus</i> and <i>Strongylus vulgaris</i> in a warmblood crossbred foal in Ontario.

The Canadian veterinary journal = La revue veterinaire canadienne·2026

Same author

A Randomized Controlled Trial Comparing Soy-Pea Protein to Animal Protein in Adults with Crohn's Disease.

medRxiv : the preprint server for health sciences·2026

Same author

Influence of gastrointestinal nematode species on the salivary anti-CarLA IgA response in Ontario ewe lambs.

Veterinary parasitology·2026

Same author

Verminous mastitis caused by <i>Cephalobus cubaensis</i> infection in a mare.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2026

Same author

Prevalence and distribution of <i>Echinococcus</i> species in domestic dogs and wild canids in Pennsylvania: KeyScreen® GI Parasite PCR testing of fecal samples.

One health (Amsterdam, Netherlands)·2026

Search research articles

Related Experiment Video

Updated: Jul 17, 2026

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

Clostridium difficile PCR ribotypes in calves, Canada.

Alexander Rodriguez-Palacios¹, Henry R Stämpfli, Todd Duffield

¹Department of Clinical Studies, University of Guelph, Guelph, Ontario, Canada. arodrigu@uoguelph.ca

Emerging Infectious Diseases

|February 8, 2007

Summary

Clostridium difficile (C. difficile) was found in calves, with some strains matching those in humans. This suggests cattle may act as reservoirs for C. difficile, potentially spreading it to people.

More Related Videos

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

Related Experiment Videos

Last Updated: Jul 17, 2026

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

Published on: September 29, 2017

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

Area of Science:

Veterinary Medicine
Infectious Diseases
Microbiology

Background:

Clostridium difficile (C. difficile) is a significant cause of infectious diarrhea in humans.
The potential role of cattle as reservoirs for C. difficile and its transmission to humans remains an area of investigation.

Purpose of the Study:

To investigate the prevalence of C. difficile in calves.
To compare bovine and human C. difficile PCR ribotypes.
To assess the association between C. difficile and calf diarrhea.

Main Methods:

A case-control study was conducted on 102 Canadian dairy farms.
Fecal samples from diarrheic and control calves were cultured for C. difficile.
ELISA was used to detect C. difficile toxins A and B.
PCR ribotyping was performed on C. difficile isolates.

Main Results:

C. difficile was isolated from 11.2% of calves overall, with a higher prevalence in control calves (14.9%) than diarrheic calves (7.6%).
C. difficile toxins were detected in 39.6% of diarrheic calves and 20.9% of control calves.
PCR ribotyping identified 8 distinct patterns, 7 of which are found in humans, including two linked to severe human disease (PCR types 017 and 027).

Conclusions:

C. difficile may be associated with calf diarrhea.
Bovine C. difficile strains share similarities with human strains, indicating cattle could be reservoirs for C. difficile transmission to humans.