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Structural studies of racecadotril and its process impurities by NMR and mass spectroscopy
K Mallikarjun Reddy1, J Moses Babu, P Sudhakar
1Department of Analytical Research, Discovery Research, Dr. Reddy's Laboratories Ltd., Miyapur, Hyderabad, India.
Abstract:
Three unknown impurities in racecadotril bulk drug at levels below 0.5% were detected by simple reverse phase isocratic high performance liquid chromatography (HPLC). Structures for these impurities were proposed by molecular ion information and their fragmentation pattern obtained by LC-MS and these impurities were confirmed by NMR spectroscopy. The impurities I, II and III were characterized as benzyl 2-methyl carboximido acetate, benzyl 2-phenyl ethyl carboximido acetate, and benzyl 2-(1-benzyl vinyl carboximido) acetate. These structures were further confirmed by co-injecting of synthetic standards of impurities with racecadotril. The mechanism of the formation of these process related impurities is discussed.
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