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Augmentation of antigen-specific antibody production and IL-10 generation with a fraction from Rooibos (Aspalathus

Kenji Ichiyama1, Akihiro Tai, Itaru Yamamoto

  • 1Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Okayama, Japan.

Bioscience, Biotechnology, and Biochemistry
|February 8, 2007
PubMed

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...