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[Comparative study of two methods of DNA extraction from different colour costal cartilage in STR genotyping]
1Shanghai Institute of Criminal Science and Technology, Shanghai 200083, China.
Objective:
To compare the effectiveness of two methods of DNA extraction from different colour costal cartilages in STR genotyping.
Methods:
DNA fragments were extracted from costal cartilages of different colour of 30 corrupt corpses using Chelex-100 and Phenol-Chloroform methods. STR loci were analyzed by ABI 3100 genetic analyzer after PCR amplification by using Profiler Plus kit.
Results:
STR loci were completely detected in all 30 samlpes of costal cartilages when using Phenol-Chloroform DNA extraction methods. While using Chelex-100 methods, all STR loci were identified in 22 cases (11 white, 8 light-yellow and 3 yellow cases). Partial STR loci were detected in 7 cases (3 yellow and 4 yellow-brown cases) and none in 1 brown-black cases.
Conclusion:
Suitable DNA extracion methods will be chosen depends on the colour of costal cartilage. Phenol-Chloroform methods is more effective in STR genotyping especially in dark colour costal cartilage cases.
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