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Published on: September 26, 2016
[Study on extraction process of polysaccharide from Sargassum fusiforme by enzymatic treatment]
Hua-fang Zhang1, Jing-shun Jin, Rong-mei Tan
1School of Medicine, Shaoxing University, Shaoxing 312000, China. huafang_zhang@hotmail.com
Objective:
To investigate the optimal extraction process of polysaccharides from S. fusiforme by enzymatic treatment.
Method:
The optimum extraction conditions were obtained by the experiment with the orthogonal design. The content of polysaccharides of S. fusiforme was determined by spectraphotometry.
Result:
The amount of enzyme and temperature significantly affected total polysaccharides of S. fusiforme.
Conclusion:
The optimum extraction conditions include the addition of 1. 2 x 10 (4) U x 100 g(-1) enzyme into water at pH 4. 5, and the subsequent treatment for 10 min while the temperature is maintained at 45 degrees C.
