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Updated: Jul 17, 2026
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
Published on: January 14, 2017
[Establishment of immortal lymphoblastoid cell bank of keloids pedigree]
Mei Song1, Jian-hua Gao, Xin Yan
1Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Objective:
To provide perpetual research materials for long term studies by establishing immortal lymphoblastoid cell bank of keloids pedigree.
Methods:
The immortal lymphoblastoid cell lines of keloids pedigree were established by Epstein-Barr virus transformation of peripheral blood B lymphocytes.
Results:
27 immortal lymphoblastoid cell lines of keloids pedigree were obtained successfully, all of the immortal lymphoblastoid cell lines were successfully revivificated after been frozen in liquid nitrogen.
Conclusions:
It is important to establish immortal lymphoblastoid cell bank of keloids pedigree and provide long-term DNA materials for deep study of keloids in the future.
