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Updated: Jul 17, 2026

Bioluminescent Bacterial Imaging In Vivo
Published on: November 4, 2012
Bioluminescent signal system: bioluminescence immunoassay of pathogenic organisms
1Institute of Biophysics, Russian Academy of Sciences Siberian Branch, Krasnoyarsk, 660036, Russia. lfrank@yandex.ru
Abstract:
The Ca(2+)-regulated photoprotein obelin has been examined as a label for bioluminescence immunoassay of infective agents. The hepatitis B virus (HbsAg) and the bacteria Escherichia coli and Shigella sonnei lipopolysaccharide (LPS) were chosen as model antigens. Chemically synthesized obelin-corresponding antibody conjugates were used in a solid-phase microplate immunoassay. The sensitivities achieved by the assay were 0.25 ng/mL for S. sonnei LPS and 0.375 ng/mL for HbsAg. A novel, filter-based immunoassay to determine bacterial admixtures in the environment was proposed. The NanoCeram filters were effectively applied to 'trap' and pre-concentrate pathogens from samples under study for the purposes of further detection and measurement of the absorbed material by bioluminescence immunoassay.
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