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Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

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Size-selective colloidal-gold localization in transmission X-ray microscopy.

H Stollberg¹, P Guttmann, P A C Takman

¹Biomedical and X-Ray Physics/Department of Applied Physics, KTH-AlbaNova, 10691 Stockholm, Sweden. heide.stollberg@biox.kth.se

Journal of Microscopy

|February 9, 2007

Summary

A new image-analysis algorithm precisely localizes small colloidal gold nanoparticles for advanced X-ray microscopy. This method enables size-selective identification, crucial for functional imaging and protein localization studies.

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Published on: February 5, 2016

Area of Science:

Materials Science
Biophysics
Microscopy

Background:

Colloidal gold nanoparticles are valuable for functional imaging in transmission X-ray microscopy.
Low contrast of small gold particles necessitates advanced localization algorithms.

Purpose of the Study:

To develop a robust algorithm for identifying and localizing single colloidal gold particles.
To enable size-selective detection down to 50 nm diameter.

Main Methods:

An image-analysis algorithm combining local absorption thresholding and Gaussian profile fitting for shape discrimination.
Application to transmission X-ray microscopy data.

Main Results:

Successful size-selective localization of single colloidal gold particles down to 50 nm.
Demonstrated potential for high-resolution imaging applications.

Conclusions:

The developed algorithm enhances the utility of colloidal gold as a marker in X-ray microscopy.
Potential applications include simultaneous protein localization and fiducial marker analysis in X-ray tomography.