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Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers
Akinobu Kajikawa1, Eiichi Satoh, Rob J Leer
1Division of Biomedical Food Research, National Institute of Health Sciences, Tokyo, Japan. kajikawa@nihs.go.jp
Abstract:
A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in either feces or sera but induced the release of IFN-gamma on restimulation of primed lymphocytes ex vivo. The results suggested that the protective efficacy provided by flagellin-expressing L. casei is mainly attributable to cell-mediated immune responses. In addition, an adjuvant-type effect of the antigen delivery system with L. casei was also observed.
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