Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial Intelligence for CELLSEARCH Image Analysis.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Longitudinal Analysis of Manually and Automatically Classified Circulating Tumor Biomarkers and their Prediction of Survival in Metastatic Colorectal Cancer.

Clinical pharmacology and therapeutics·2025
Same author

PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients.

Cancer research communications·2025
Same author

Design, molecular characterization and therapeutic investigation of a novel CCR8 peptide antagonist that attenuates acute liver injury by inhibiting infiltration and activation of macrophages.

Acta pharmaceutica Sinica. B·2025
Same author

Performance comparison of streptavidin magnetic beads for epcam expressing cancer cell lines for circulating tumor cell (CTC) enrichment in a flow-through immunomagnetic system.

PloS one·2025
Same author

Clinical Validity of Repeated Circulating Tumor Cell Enumeration as an Early Treatment Monitoring Tool for Metastatic Breast Cancer in the PREDICT Global Pooled Analysis.

Clinical cancer research : an official journal of the American Association for Cancer Research·2025

Related Experiment Video

Updated: Jul 17, 2026

Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation
05:25

Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation

Published on: May 17, 2021

An immunomagnetic single-platform image cytometer for cell enumeration based on antibody specificity.

Xiao Li1, Arjan G J Tibbe, Erik Droog

  • 1Biophysical Engineering Group, University of Twente, Dienstweg 1, Building Zuidhorst, 7522 ND Enschede, The Netherlands. l.x.lixiao@tnw.utwente.nl

Clinical and Vaccine Immunology : CVI
|February 9, 2007
PubMed
Summary

A new immunomagnetic single-platform image cytometer (SP ICM) offers a simple, affordable method for cell enumeration. This technology shows promise for accurate blood cell counting in resource-poor settings.

More Related Videos

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Related Experiment Videos

Last Updated: Jul 17, 2026

Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation
05:25

Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation

Published on: May 17, 2021

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Cell Biology

Background:

  • Accurate cell enumeration is crucial for disease diagnosis and monitoring.
  • Existing technologies like flow cytometry can be expensive and complex, limiting their use in resource-poor settings.
  • There is a need for simplified, cost-effective cell counting methods.

Purpose of the Study:

  • To develop and evaluate an immunomagnetic single-platform image cytometer (SP ICM) for cell enumeration.
  • To assess the system's precision, linearity, and correlation with established flow cytometry methods.
  • To explore the potential of SP ICM for enumerating specific cell populations, including T lymphocytes.

Main Methods:

  • An immunomagnetic single-platform image cytometer (SP ICM) was designed using antibody specificity for cell labeling.
  • Cells were stained with acridine orange and magnetically labeled, concentrating them for image capture.
  • The system was validated by enumerating leukocytes (CD45), T lymphocytes (CD3), and B lymphocytes (CD19), comparing results with SP and DP flow cytometry.

Main Results:

  • The SP ICM demonstrated excellent precision and linearity in cell enumeration.
  • Cell counts obtained by SP ICM showed good correlation (R >= 0.95) with both SP (TruCount) and dual-platform (DP) flow cytometry methods.
  • The system showed higher counts for CD4(+) cells due to the inclusion of CD4(+) dim monocytes, indicating potential for broader immune cell profiling.

Conclusions:

  • The developed immunomagnetic SP ICM is a simple and potentially affordable technology for cell enumeration.
  • The system shows promise for accurate blood cell counting, particularly in resource-limited environments.
  • Further development with additional labels, like CD3, could enable precise enumeration of specific lymphocyte subsets such as CD4(+) T lymphocytes.