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Updated: Jul 17, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Multi-dimensional fingerprints of Japan Jiuxin pill]
Min Zhang1, Yi-Ming Wang, Guo-An Luo
1Jiangxi College of Traditional Chinese Medicine, Nanchang 330006, China.
Aim:
To develop HPLC-UV/ELSD and HPLC-UV/MS3 fingerprints of Japan Jiuxin pill using multi-dimensional hyphenated methods and study on its chemical base.
Methods:
Separation was performed on Altima C18 analytical column (250 mm x 4.6 mm ID, 5 microm). The mobile phases consisted of water containing 0.2% formic acid and acetonitrile was used as gradient elute. The flow rate was 1.0 mL x min(-1). An electrospray ion trap mass spectrometer was utilized for qualitative analysis and both positive and negative secondary ion scan mode were applied.
Results:
HPLC-UV/ELSD and HPLC-UV/ MS3 fingerprints of Japan Jiuxin pill with well separation and reproducibility were obtained. Totally 21 components in the complex formula were identified and the chemical structural information of glycocholic acid and its isomer were suggested.
Conclusion:
The research is propitious to offer an effective and reliable pattern for analyzing Japanese Kampo Medicines as well as its quality control.
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