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Updated: Jul 17, 2026

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
Published on: June 19, 2018
[Advances of study on human translesion synthesis DNA polymerase eta]
Gong-hua Hu1, Zhi-xiong Zhuang
1Department of Preventive Medicice, School of Public Heath, Sun Yat-sen University, Guangzhou 510080, China.
Abstract:
Translesion synthesis(TLS) is a mechanism of DNA damage tolerance in cells,which can be divided into two categories: error-free TLS and error-prone TLS. Human polymerase eta( Pol eta) is one of translesion synthesis DNA polymerases. This protein exhibits lower fidelity and processivity than replicative DNA polymerases. In addition, it possesses "action-at-a-distance" and reverse transcriptase activity. Human Pol eta has the ability to catalyze error-free TLS and error-prone TSL, and may be involved in somatic hypermutation of immunoglobulin variable genes.
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