Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selective tumor cell targeting using low-affinity, multivalent interactions.

Coby B Carlson1, Patricia Mowery, Robert M Owen

  • 1Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.

ACS Chemical Biology
|February 13, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemical Probes to Reveal the Assembly and Dynamics of Wall Teichoic Acids.

Journal of the American Chemical Society·2026
Same author

PBRM1-VHL cooperation rewires lipid and iron metabolism to promote ferroptosis resistance in clear cell renal cell carcinoma.

bioRxiv : the preprint server for biology·2026
Same author

Management of Cardiac Implantable Electronic Devices in the Ambulatory Setting.

Anesthesiology clinics·2026
Same author

Structural landscape of H3K27me3 recognition by protein domains and their potential for inhibition.

The Journal of biological chemistry·2026
Same author

Principles of <i>in situ</i> protein sequencing: expansion microscopy-adapted Edman degradation and amino acid recognition.

bioRxiv : the preprint server for biology·2026
Same author

Covalent Protein Inhibitors via Tyrosine and Tryptophan Conjugation with Cyclic Imine Mannich Electrophiles.

Angewandte Chemie (International ed. in English)·2026
Same journal

Lipid Metabolic Labeling to Study Site- and Lipid-Specific Long-Chain <i>S</i>-Acylation Dynamics.

ACS chemical biology·2026
Same journal

Inositol Thiophosphates as Inhibitors of Mammalian, Plant, and Fungal Phytases.

ACS chemical biology·2026
Same journal

Synthesis and Characterization of the Spectroscopic and Imaging Utilities of Two Indole-Based Cyan Fluorescent Nucleoside Analogues.

ACS chemical biology·2026
Same journal

Indole Ring Expansion and Rearrangement-Enabled Quinoline Scaffold Formation in the Biosynthesis of the Antitumor Monoterpene Indole Alkaloid Camptothecin.

ACS chemical biology·2026
Same journal

Intracellular Delivery of Peptides and Proteins with an Engineered Membrane Translocation Domain.

ACS chemical biology·2026
Same journal

Development of Next-Generation Fluoroacetamidine-Containing Activity-Based Probes for the Selective Labeling of the Protein Arginine Deiminases (PADs).

ACS chemical biology·2026
See all related articles

Multivalent interactions offer superior cell targeting by exploiting natural recognition processes. This strategy selectively kills tumor cells expressing high integrin levels, unlike traditional high-affinity methods.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Tumor cells often exhibit distinct cell surface receptor expression compared to normal cells.
  • Selective targeting of cancer cells is crucial for effective therapeutic strategies.
  • Traditional high-affinity targeting methods can lack specificity, leading to off-target effects.

Purpose of the Study:

  • To develop a novel strategy for selective tumor cell killing using multivalent interactions.
  • To investigate the efficacy of a bifunctional small-molecule ligand for targeted cell destruction.
  • To compare the selectivity of multivalent targeting with traditional high-affinity methods.

Main Methods:

  • Synthesized a bifunctional small-molecule ligand with an Arg-Gly-Asp (RGD) peptidomimetic and an alpha-galactose (alpha-Gal) epitope.

Related Experiment Videos

  • RGD motif targets alphavbeta3 integrins, while the alpha-Gal epitope binds anti-Gal antibodies.
  • Assessed cell lysis triggered by anti-Gal antibody recruitment to cells with high alphavbeta3 integrin expression.

    • Main Results:

    • Multivalent presentation of alpha-Gal epitopes on cells with high alphavbeta3 integrin levels potently recruited anti-Gal antibodies.
    • This recruitment triggered complement-mediated lysis, selectively killing targeted cells.
    • In contrast, doxorubicin tethered to the RGD ligand resulted in indiscriminate cell death.

    Conclusions:

    • Low-affinity, multivalent interactions provide a highly selective method for distinguishing and targeting specific cell types.
    • This strategy demonstrates superior selectivity compared to traditional abiotic, high-affinity targeting approaches.
    • The findings have significant implications for developing advanced cancer therapies and treating other diseases with aberrant cell populations.