Related Experiment Video
Updated: Jul 17, 2026

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
Published on: November 21, 2013
Double assembly composed of lectin association with columnar molecular assembly of cyclic tri-beta-peptide having
Futoshi Fujimura1, Yoshiki Horikawa, Tomoyuki Morita
1Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Abstract:
A novel double assembly was prepared by association between a columnar molecular assembly of cyclic tri-beta-peptides having sugar units and lectins. The NMR, FT-IR, and circular dichroism (CD) spectroscopy as well as computational calculations revealed that this compound took a flat and C3 symmetrical conformation and that the amide N-H and C=O groups protruded vertically to the ring plane. This disk-shaped molecule stacked one by one to form a columnar structure via intermolecular hydrogen bonds between the amide groups. WGA lectin moderately bound to this columnar assembly to form a double assembly. Another lectin (Con A) disturbed the columnar structure upon strong binding, and RCA lectin showed no binding. Fluorescence spectroscopy revealed that the association between WGA lectin and columnar assembly of cyclic glycopeptide could be achieved due to the high density of the hydroxyl groups on the assembly surface (cluster effects). Interestingly, after cross-linking the lectins bound to the columnar assembly (the double assembly) by glutaraldehyde, the core column of cyclic tri-beta-peptides could be washed away to leave the protein nanotube.
More Related Videos
09:54Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides
Published on: August 20, 2018
08:14Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
Published on: February 14, 2013
Related Concept Videos
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Protein Complex Assembly
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Proteoglycans
Laminins are the Adhesive Proteins of Basal Lamina
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
Protein Glycosylation
Glycosylation occurs in...
Assembly of Signaling Complexes
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...