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The DNA Helix01:16

The DNA Helix

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The DNA Helix01:07

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Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
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Updated: Jul 17, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
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Published on: April 12, 2019

Cylindrical poly(oligo-DNA).

Sabine Fluegel1, Michael Maskos

  • 1Institute of Physical Chemistry, University Mainz, Welder Weg 11, D-55128 Mainz, Germany.

Biomacromolecules
|February 13, 2007
PubMed
Summary

Researchers created polymerizable oligo-nucleic acids (ODNs) that self-assemble into cylindrical poly(oligo-DNA) structures. These unique structures exhibit a semi-flexible wormlike shape in solution, confirmed by light scattering and atomic force microscopy.

Area of Science:

  • Polymer Chemistry
  • Biomaterials Science
  • Nanotechnology

Background:

  • Oligo-nucleic acids (ODNs) are short DNA sequences with potential applications in nanotechnology and medicine.
  • Modifying ODNs to enable polymerization is crucial for creating novel biomaterials.
  • Understanding the self-assembly behavior of modified ODNs is key to designing functional nanomaterials.

Purpose of the Study:

  • To synthesize polymerizable ODNs by functionalizing a specific ODN sequence.
  • To investigate the self-assembly and structural properties of the resulting polymers.
  • To characterize the shape and dimensions of the poly(oligo-DNA) in solution and on surfaces.

Main Methods:

  • Chemical modification of 5'-TCC ATG ACG TTC-3' ODNs with an amine group and N-methacryloyloxysuccinimide.

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  • Free radical homopolymerization to form comb polymers with ODN side-chains.
  • Static and dynamic light scattering (SLS/DLS) for solution characterization.
  • Atomic force microscopy (AFM) for surface visualization.

    • Main Results:

    • Successful synthesis of polymerizable ODNs.
    • Formation of comb polymers with ODN side-chains, adopting a semi-flexible wormlike main chain conformation due to steric repulsion.
    • Characterization revealed cylindrical poly(oligo-DNA) molecules with Rg,app = 67.8 nm and Rh,app = 44.6 nm, indicating a characteristic ratio rho = 1.52.
    • AFM confirmed the cylindrical morphology of adsorbed poly(oligo-DNA) on mica.

    Conclusions:

    • The steric repulsion in comb polymers forces a semi-flexible wormlike chain, leading to cylindrical poly(oligo-DNA) structures.
    • Poly(oligo-DNA) molecules exhibit a distinct cylindrical shape in aqueous solution.
    • The study demonstrates a method for creating novel cylindrical nanostructures using modified ODNs, with potential for advanced material applications.