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Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
Published on: November 28, 2019
Localization of protein secretion in fungal colonies using a novel culturing technique; the ring-plate system
Ana M Levin1, Ronald P de Vries, Han A B Wösten
1Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Journal of Microbiological Methods
|February 13, 2007
Abstract:
A novel culturing technique, called the ring-plate system, is described. Fungal colonies are grown on a polycarbonate membrane placed on a plate with 6 ring-shaped wells that are filled with liquid medium. This culturing technique enables simultaneous monitoring of environmental conditions and secretion in different parts of the fungal colony.
