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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...

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Related Experiment Video

Updated: Jul 17, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Recent advances in plant recombination.

Jin Li1, An-Ping Hsia, Patrick S Schnable

  • 1Brandeis University, Waltham, Massachusetts 02454-9110, USA.

Current Opinion in Plant Biology
|February 13, 2007
PubMed
Summary

Genetic recombination is vital for plant diversity. Studies reveal factors influencing recombination rates and breakpoints, paving the way for precise gene targeting in plants.

Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Recombination is a fundamental cellular process driving genetic diversity.
  • Factors like DNA sequence similarity and transposon activity influence recombination rates and breakpoint distribution in plants.
  • Understanding interchromatid and interhomolog recombination is crucial for genetic studies.

Purpose of the Study:

  • To explore the impact of various factors on recombination rates and breakpoint patterns in plants.
  • To characterize interchromatid and interhomolog recombination events in detail.
  • To highlight advancements in gene targeting approaches for plants.

Main Methods:

  • Analysis of recent studies on plant recombination.
  • Characterization of recombination breakpoints.

More Related Videos

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Related Experiment Videos

Last Updated: Jul 17, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

  • Review of emerging gene targeting technologies.

    • Main Results:

    • Identified key factors affecting recombination rates and distribution, including DNA sequence similarity and transposon activation.
    • Provided detailed descriptions of interchromatid and interhomolog recombination mechanisms.
    • Highlighted the potential of new methods for achieving gene targeting in plants.

    Conclusions:

    • Recombination is a complex process influenced by multiple factors in plants.
    • Advances in understanding recombination are critical for developing effective gene targeting strategies.
    • New approaches hold significant promise for future plant genetic engineering.