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Updated: Jul 17, 2026
Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
Published on: March 12, 2019
Methylprednisolone administration alters adenine nucleotide hydrolysis in rat blood serum
Iraci Lucena S Torres1, Cristina Ribas Fürstenau, Gabriela Rossi
1Departamentos de Farmacologia, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Sarmiento Leite 500, 90050-170 Porto Alegre, RS, Brazil. iracitorres@gmail.com
Abstract:
The effect of methylprednisolone on the hydrolysis of adenine nucleotides by rat blood serum enzymes was studied. Adult male Wistar rats were submitted to three different treatments with synthetic steroid methylprednisolone: one dose of 50 mg/kg, i.p. (acute); or oral doses of 6 mg/kg dissolved in drinking water for 15 (sub-chronic) or 30 (chronic) days. Decreased ADP hydrolysis was observed after acute and sub-chronic treatments. Furthermore, ATP, ADP and AMP hydrolysis decreased after chronic treatment. These alterations may constitute one of the mechanisms that mediate the development of some of the side effects associated with corticosteroid use.
