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Updated: Jul 17, 2026

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Published on: July 10, 2021

Vitrification solution containing DMSO and EG can induce parthenogenetic activation of in vitro matured ovine oocytes

Shu-Jun Tian¹, Chang-Liang Yan, Hui-Xin Yang

¹College of Animal Science and Technology, and State Key Laboratories for Agricultural Biotechnology, China Agricultural University, Beijing, PR China.

Animal Reproduction Science

|February 13, 2007

Summary

Vitrification solutions (VS) containing DMSO and EG can cause parthenogenetic activation in ovine oocytes, leading to reduced fertilization rates. This study investigated the mechanisms behind decreased monospermy in vitrified ovine oocytes.

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Last Updated: Jul 17, 2026

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Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

Area of Science:

Reproductive Biology
Cryobiology
Oocyte Maturation

Background:

Vitrification is a key technique in cryopreservation of oocytes.
Reduced fertilization rates have been observed in vitrified ovine oocytes.
The underlying mechanisms require further investigation.

Purpose of the Study:

To examine the reduced incidence of normal fertilization in vitrified ovine oocytes.
To investigate the effects of vitrification solution (VS) on oocyte activation and sperm penetration.
To elucidate the mechanisms responsible for decreased monospermy.

Main Methods:

Ovine oocytes were matured in vitro and allocated to control, toxicity (VS exposure), or vitrification groups.
Sperm penetration and monospermy rates were assessed after in vitro fertilization.
Cortical granule (CG) release, zona pellucida (ZP) resistance to pronase E, and female pronucleus formation were analyzed.

Main Results:

Monospermy rates significantly decreased in VS-exposed and vitrified oocytes compared to controls.
Increased CG release and ZP hardening (longer pronase E digestion time) were observed in treated oocytes.
Parthenogenetic activation, indicated by increased female pronucleus formation, occurred in VS-exposed and vitrified oocytes.

Conclusions:

Vitrification solutions containing DMSO and EG can induce parthenogenetic activation in ovine oocytes.
This activation leads to ZP hardening and reduced sperm penetration, explaining the decreased fertilization rates.
Understanding these effects is crucial for optimizing ovine oocyte cryopreservation protocols.