Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput oncogene mutation profiling in human cancer.

Roman K Thomas1, Alissa C Baker, Ralph M Debiasi

  • 1Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, USA.

Nature Genetics
|February 13, 2007
PubMed

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CRISPR base editor screening identifies spectrum of MEN1 mutations impacting menin inhibitors in clinical trials.

Nature communications·2026
Same author

CDKN1B inactivation impacts ER signaling and drives resistance to endocrine therapy in breast cancer.

British journal of cancer·2026
Same author

High-throughput ligand diversification to discover chemical inducers of proximity.

Nature chemical biology·2026
Same author

Correction: Enhanced proteasomal activity is essential for long term survival and recurrence of innately radiation resistant residual glioblastoma cells.

Oncotarget·2025
Same author

High-throughput diversification of protein-ligand surfaces to discover chemical inducers of proximity.

bioRxiv : the preprint server for biology·2025
Same author

Genomic characterization of AI resistance in hormone receptor-positive breast cancer using LTED cell line models.

Biochemical and biophysical research communications·2025
Summary
This summary is machine-generated.

Researchers developed a high-throughput method to detect oncogene mutations in 1,000 tumors across 17 cancer types. This approach identified new mutations and co-occurrences, aiding real-time cancer classification and treatment.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Understanding tumor genetics is crucial for cancer treatment.
  • Current methods lack the sensitivity and scalability to identify critical genetic events in diverse human tumors.

Purpose of the Study:

  • To adapt high-throughput genotyping for analyzing known oncogene mutations in a large cohort of human tumors.
  • To establish mutation profiles across various cancer types and identify novel genetic alterations.

Main Methods:

  • High-throughput genotyping was employed to screen 238 known oncogene mutations.
  • 1,000 human tumor samples representing 17 distinct cancer types were analyzed.

Main Results:

  • Mutations were detected in 14 out of 17 analyzed oncogenes, with 30% of samples harboring at least one mutation.

Related Experiment Videos

  • Previously unrecognized oncogene mutations were identified in several tumor types.
  • A high frequency of co-occurring oncogene mutations was observed.

    • Conclusions:

    • High-throughput genotyping enables simultaneous interrogation of multiple cancer genes in real time.
    • This approach enhances tumor classification and supports the development of targeted cancer therapies.