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Modulation of transforming growth factor beta2 (TGF-beta2) by inositol hexaphosphate in colon carcinogenesis in rats
Guido Marks1, Ricardo Dutra Aydos, Djalma José Fagundes
1Department of Surgery, UFMS, Brazil. gmarks@brturbo.com.br
Purpose:
To evaluate modulation in the expression of Transforming growth factor beta2 (TGF-beta2) in short-term colon carcinogenesis.
Methods:
64 male rats was used, comprising 4 groups of 16 animals each: group 1 received Inositol hexaphosphate (IP6) and azoxymethane (AOM); group 2, AOM alone; group 3, IP6 alone; group 4 was used as control. Groups 1 and 3 were given 1% IP6 in drinking water for 6 weeks. AOM was administered subcutaneously at weeks 3 and 4 of the experiment at 20 mg/kg of body weight each week. Immunohistochemical processing was performed with the use of anti-TGF-beta2 primary antibodies in right colon samples and quantitation of TGF-beta2 as percentage of expression, through computer-assisted image processing.
Results:
mean values of TGF-beta2 expression were 9.0 +/- 3.9% for group 4 (control), 12.7 +/- 4.0% for group 3 (IP6), 19.3 +/- 6.2% for group 2 (AOM), and 13.1 +/- 5.3% for group 1 (IP6+AOM). The value of p was calculated as 0.0001 for a 5% or lower significance level.
Conclusion:
the experiment revealed a significant increase in TGF-beta2 expression in right colon with the administration of AOM, and a significant decrease in TGF-beta2 expression when IP6 was administered with AOM.
Insights
This study shows that azoxymethane (AOM) increases Transforming Growth Factor beta2 (TGF-beta2) expression in colon cancer. However, Inositol hexaphosphate (IP6) can reduce this TGF-beta2 increase when used with AOM.
Area of Science:
- Oncology
- Gastroenterology
- Biochemistry
Background:
- Transforming Growth Factor beta2 (TGF-beta2) plays a role in carcinogenesis.
- Colon carcinogenesis involves complex molecular changes.
- Dietary compounds are investigated for cancer chemoprevention.
Purpose of the Study:
- To investigate the effect of Inositol hexaphosphate (IP6) on Transforming Growth Factor beta2 (TGF-beta2) expression during short-term colon carcinogenesis.
- To evaluate the modulation of TGF-beta2 in a rat model induced by azoxymethane (AOM).
Main Methods:
- A rat model of short-term colon carcinogenesis was established using azoxymethane (AOM).
- Animals were divided into four groups: control, AOM alone, IP6 alone, and AOM + IP6.
- Immunohistochemistry and computer-assisted image processing were used to quantify TGF-beta2 expression in colon tissue.
Main Results:
- Azoxymethane (AOM) administration significantly increased TGF-beta2 expression in the right colon.
- Inositol hexaphosphate (IP6) administration alone showed a slight, non-significant increase in TGF-beta2 expression.
- Co-administration of IP6 with AOM significantly reduced the AOM-induced increase in TGF-beta2 expression.
Conclusions:
- Azoxymethane (AOM) significantly upregulates TGF-beta2 expression, suggesting its involvement in AOM-induced colon carcinogenesis.
- Inositol hexaphosphate (IP6) demonstrates chemopreventive potential by mitigating the increase in TGF-beta2 expression caused by AOM.
- These findings highlight TGF-beta2 as a potential biomarker and therapeutic target in colon cancer prevention strategies involving IP6.
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