Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electropermeabilization of dense cell suspensions.

Gorazd Pucihar1, Tadej Kotnik, Justin Teissié

  • 1Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, 1000 Ljubljana, Slovenia.

European Biophysics Journal : EBJ
|February 13, 2007
PubMed
Summary

Cell density significantly impacts electropermeabilization. Higher cell densities reduce the fraction of permeabilized cells and dye loading due to altered electric fields and cell swelling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intravascular Hemolysis and Pulsed-Field Ablation: State of the Art Review.

JACC. Clinical electrophysiology·2026
Same author

Modulation of Contactless High Intensity Pulsed Electromagnetic Field Induced Electroporation and Gene Delivery Efficacy Using Various Nanoparticles.

Bioelectricity·2026
Same author

Pulsed field ablation for the interventional treatment of atrial fibrillation: a scientific statement of the European Heart Rhythm Association of the European Society of Cardiology, the Heart Rhythm Society, the Asia Pacific Heart Rhythm Society, the Latin American Heart Rhythm Society, and the Canadian Heart Rhythm Society.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2026
Same author

2026 HRS/EHRA scientific statement on pulsed field ablation for cardiac arrhythmias.

Heart rhythm·2026
Same author

Pulsed field ablation: Disrupting technology in cardiac electrophysiology.

Heart rhythm·2025
Same author

Complexity and Perplexity of Pulsed Field Ablation: An Engineering Perspective.

Arrhythmia & electrophysiology review·2025

Area of Science:

  • Biophysics
  • Cell Biology
  • Biotechnology

Background:

  • Cell membrane electropermeabilization is crucial for drug delivery and gene therapy.
  • Understanding factors influencing electropermeabilization efficiency is vital for optimizing these applications.
  • Dense cell suspensions serve as a relevant model for tissue electropermeabilization.

Purpose of the Study:

  • To investigate the effect of cell density on cell membrane electropermeabilization.
  • To quantify the relationship between cell density and permeabilization efficiency.
  • To elucidate the mechanisms behind density-dependent changes in electropermeabilization.

Main Methods:

  • Experiments conducted on dense cell suspensions (up to 400 x 10^6 cells/ml).
  • Assay of permeabilization using a fluorescence test with Propidium iodide.

Related Experiment Videos

  • Measurement of mean number of permeabilized cells and mean fluorescence per cell.

    • Main Results:

    • Fraction of permeabilized cells decreased by approximately 50% as cell density increased from 10 x 10^6 to 400 x 10^6 cells/ml.
    • Higher pulse amplitude required for equivalent permeabilization in dense suspensions.
    • Electroloading decreased with cell density, partly due to cell swelling and slower resealing.

    Conclusions:

    • Cell density significantly influences electropermeabilization efficiency by altering local electric fields and transmembrane voltage.
    • Cell swelling post-permeabilization in dense suspensions hinders dye uptake and loading.
    • Slower cell resealing in dense suspensions may also contribute to reduced electroloading.