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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
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Updating the metagenomics toolbox.

Esther Gabor1, Klaus Liebeton, Frank Niehaus

  • 1BRAIN Aktiengesellschaft, Zwingenberg, Germany.

Biotechnology Journal
|February 13, 2007
PubMed
Summary

Metagenomics is advancing with new technologies for analyzing uncultivated microbes. Challenges remain, especially in expressing enzymes from these organisms.

Area of Science:

  • Microbiology
  • Genomics
  • Molecular Biology

Background:

  • Metagenomics, the study of microbial communities through genomic analysis, is rapidly evolving.
  • Uncultivated microorganisms represent a vast, largely untapped resource of molecular and functional diversity.

Purpose of the Study:

  • To highlight recent technological advancements in metagenomics.
  • To identify current challenges and future directions in the field.

Main Methods:

  • Randomly proliferating limited-source DNA amplification.
  • Massively parallel sequencing without cloning.
  • Isolation of target sequences from complex mixtures.
  • High-throughput assays for metabolic pathways.
  • cDNA cloning from extracted mRNA for active gene discovery.

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Published on: April 4, 2018

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A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
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A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Main Results:

  • Significant progress in DNA amplification, sequencing, and target isolation techniques.
  • Development of high-throughput systems for functional analysis and gene expression.
  • Successful direct cloning of actively expressed genes from microbial consortia.

Conclusions:

  • Metagenomic technologies are maturing, enabling deeper exploration of microbial diversity.
  • Efficient heterologous expression of enzymes from uncultured organisms remains a key challenge.