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Published on: May 16, 2012
[Quantification of PPARdelta mRNA by real-time RT-PCR in rectal cancer tissues]
Lie Yang1, Yong-yang Yu, Zong-guang Zhou
1Division of Digestive Surgery and Organ-Microcirculation, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To clarify the expression change of PPARdelta gene in human rectal cancer tissues and determine the correlation of PPARdelta expression with the clinical and pathological parameters of rectal cancer.
Methods:
Applying real-time RT-PCR, we quantified PPARdelta mRNA in 86 tissues from excised primary rectal cancers. In each case, accompanying normal mucosa was collected for comparison.
Results:
Among the 86 rectal cancer tissues, 48 (55.8%) cases showed PPARdelta overexpression: 39 (81.3%) tumors gave an expression level 1.5 to 5.0 times, 5 (10.4%) tumors 10 to 20 times, and 4 (8.3%) tumors more than 20 times relative to normal mucosa. However, the general level of PPARdelta mRNA in rectal cancer tissues is not statistically different from that in normal mucosa. There was no evidence for the relationships of PPARdelta expression with cell differentiation, pathological categories and Dukes stages.
Conclusion:
The expression of PPARdelta gene in rectal cancers is not statistically different from that in normal mucosa, and it is not correlated with cell differentiation, pathological categories and Dukes stages.
Insights
PPARdelta gene expression in rectal cancer tissues was analyzed. Results indicate no significant difference compared to normal tissue, nor correlation with clinical or pathological parameters.
Area of Science:
- Molecular biology
- Oncology
- Gene expression analysis
Context:
- Rectal cancer is a significant global health concern.
- Understanding gene expression alterations is crucial for identifying potential therapeutic targets.
- Peroxisome proliferator-activated receptor delta (PPARdelta) is implicated in various cellular processes, including cancer development.
Purpose:
- To investigate the expression levels of PPARdelta in human rectal cancer tissues.
- To determine the correlation between PPARdelta gene expression and clinical-pathological features of rectal cancer.
Summary:
- PPARdelta mRNA levels were quantified in 86 rectal cancer tissues and adjacent normal mucosa using real-time RT-PCR.
- While some rectal tumors exhibited PPARdelta overexpression relative to normal mucosa, the overall expression level was not statistically different between cancer and normal tissues.
- No significant correlation was found between PPARdelta expression and clinicopathological parameters such as cell differentiation, pathological categories, or Dukes stages.
Impact:
- This study suggests that PPARdelta may not serve as a reliable prognostic biomarker for rectal cancer.
- Further research is needed to fully elucidate the role of PPARdelta in rectal carcinogenesis.
- Findings contribute to the ongoing efforts to understand the molecular landscape of rectal cancer.
