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[A study on the changes in phospholipids content of plasma and liver in rats with hypertriglyceridemia induced by
1Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the changes of phospholipids content in plasma and liver in rats' hypertriglyceridemia (HTG) induced by high-starch diet, and to gain an insight into the relationship of triglyceride with phospholipids.
Methods:
HTG rats were fed with high-starch diet, and the control rats with regular diet. After 6 days, triglyceride (TG), total cholesterol (TC), and blood glucose were measured by enzymatic kits. Phospholipids (PL) were measured by antiscorbutate reduction method. High density lipoproteins (HDLs) were separated by phosphotungistic acid. HDL-TG and HDL-C were determined by enzymatic kits, HDL-PL contents were measured by antiscorbutate reduction method. NHDL-TG, NHDL-C and NHDL-PL contents were calculated by minus of HDL contents from total contents. TG, TC and PL in liver were measured by chemical assays.
Results:
The fasting plasma TG, NHDL-TG, glucose and PL contents of rats fed by high-starch dietary were higher than those of the rats fed by regular diet. HDL-C, HDL-PL and HDL-PL/HDL-C ratio of HTG rats were lower than those of control rats. TG and PL in liver of HTG rats were higher than those of control rats. Correlation analyses indicated that the plasma PL of HTG rats had positive correlation with TG, negative correlation with HDL-PL, and stronger correlation with NHDL-TG when compared with that of control rats. In HTG rats, HDL-PL had negative correlation with glucose, PL, and liver PL. Furthermore, HDL-PL in HTG rats had stronger negative correlation with plasma TG and NHDL-TG as compared with those in control rats. PL in the liver of HTG rats had stronger correlations with TG in liver, compared with PL in the liver of control rats.
Conclusion:
The rats with hypertriglyceridemia induced by high-starch diet have higher PL content in plasm and liver, but lower PL content in HDL, and their correlation pattern of PL in plasm and liver with other lipids is also
