Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pullulan-dextran composite beads as bone fillers: from material design and industrial production to clinical application in oral surgery.

Frontiers in bioengineering and biotechnology·2026
Same author

SHEDs and BMSCs exhibit distinct lineage preferences in HUVECs dynamic spheroid co-cultures: vascular versus osteogenic commitment.

Bioengineering & translational medicine·2026
Same author

How the Hydrogel Scaffold's Porous Structure and Composition Control the Formation of Spheroids for Bone Tissue Engineering.

ACS biomaterials science & engineering·2026
Same author

Double-network polysaccharide hydrogel for guided tissue repair.

Biomaterials science·2025
Same author

Challenges and limitations in developing of a new maxillary standardized rat alveolar bone defect model to study bone regenerative approaches in oral and maxillofacial surgery.

Frontiers in bioengineering and biotechnology·2025
Same author

The pathophysiology of acute lung injury following intestinal ischemia-reperfusion.

American journal of physiology. Lung cellular and molecular physiology·2025

Related Experiment Video

Updated: Jul 17, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Cationized pullulan 3D matrices as new materials for gene transfer.

Aurélie San Juan1, Hanna Hlawaty, Frédéric Chaubet

  • 1Inserm, U698, Bio-ingénierie Cardiovasculaire, Université Paris 7, Paris F-75018, Université Paris 13, Villetaneuse F-93430, France.

Journal of Biomedical Materials Research. Part A
|February 14, 2007
PubMed
Summary

Researchers developed a novel biocompatible cationized pullulan matrix for gene delivery. This 3D matrix effectively transfers genes into smooth muscle cells without toxicity, showing promise for local gene therapy applications.

More Related Videos

Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers
12:30

Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers

Published on: August 12, 2014

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

Related Experiment Videos

Last Updated: Jul 17, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers
12:30

Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers

Published on: August 12, 2014

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Polymer Chemistry

Background:

  • Gene delivery systems are crucial for therapeutic applications.
  • Developing safe and effective non-viral vectors remains a challenge.
  • Cationic polysaccharides offer potential as gene delivery vehicles.

Purpose of the Study:

  • To develop a novel biocompatible cationized pullulan three-dimensional (3D) matrix for gene delivery.
  • To characterize the interaction between DNA and the cationized pullulan.
  • To evaluate the gene transfer efficiency and cytotoxicity of the developed matrix.

Main Methods:

  • Synthesis and characterization of diethylaminoethyl-pullulan (DEAE-pullulan).
  • Complexation studies of DNA with DEAE-pullulan using fluorescence quenching and gel retardation assays.
  • Fabrication of DEAE-pullulan 3D matrices crosslinked with phosphorus oxychloride.
  • In vitro gene transfer studies using cultured smooth muscle cells (SMCs) and a plasmid vector (pSEAP).
  • Assessment of gene expression and cell viability.

Main Results:

  • DEAE-pullulan effectively complexed with DNA, unlike neutral pullulan.
  • DEAE-pullulan matrices facilitated a 150-fold increase in gene expression in SMCs compared to controls.
  • The 3D matrices demonstrated efficient plasmid DNA loading, protection from DNase I degradation, and significant gene transfer.
  • No significant cell toxicity was observed with the DEAE-pullulan matrices.

Conclusions:

  • Cationized pullulan 3D matrices are effective and non-toxic biomaterials for gene delivery.
  • These matrices show significant potential for local gene transfer applications.
  • Further research into cationized pullulan-based systems for gene therapy is warranted.