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Updated: Jul 17, 2026

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Published on: October 6, 2019
TNF-alpha, TNF-beta and IL-4 gene polymorphisms in Iranian patients with multiple sclerosis
E Kamali-Sarvestani1, A Nikseresht, E Aflaki
1Department of Immunology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. immunol2@sums.ac.ir
Objectives:
To investigate the association between tumor necrosis factor-alpha (TNF-alpha) G-308A, tumor necrosis factor-beta (TNF-beta) G+252A and interleukin-4 (IL-4) C-590T polymorphisms and susceptibility to multiple sclerosis (MS) development and clinical course of the disease.
Materials And Methods:
Two hundred and seventy patients with MS and 542 sex and ethnic matched controls were enrolled in the present study. An allele-specific oligonucleotide polymerase chain reaction was used to detect the polymorphism at position -308 of the TNF-alpha gene. The genotypes of TNF-beta and IL-4 were determined by polymerase chain reaction-restriction fragment length polymorphism.
Results:
Allelic and genotypic frequencies for these polymorphisms were similar in patients with MS and population controls or among different types of the disease.
Conclusion:
The results of the present study suggest that the three mentioned functional polymorphisms are not likely to cause susceptibility to MS in the Iranian population.
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