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Updated: Jul 17, 2026

Imaging ATG9A, a Multi-Spanning Membrane Protein
Published on: June 16, 2023
Atg27 is a second transmembrane cycling protein
Wei-Lien Yen1, Daniel J Klionsky
1Life Sciences Institute, Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-2216, USA.
Autophagy utilizes transmembrane proteins like Atg9 and Atg27 to build double-membrane autophagosomes. Atg27
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Autophagy is a fundamental cellular process for degrading damaged components.
- The formation of the double-membrane autophagosome is not fully understood.
- Autophagy-related (ATG) genes encode essential proteins for this pathway.
Purpose of the Study:
- To investigate the role of the transmembrane protein Atg27 in autophagy.
- To compare the trafficking of Atg27 with Atg9.
- To explore the potential involvement of the Golgi complex in autophagy.
Main Methods:
- Characterization of Atg27 in yeast Saccharomyces cerevisiae.
- Analysis of Atg27 localization and cycling.
- Comparative analysis of Atg9 and Atg27 trafficking.
Main Results:
- Atg27 is identified as a second transmembrane protein involved in autophagy.
- Atg27 exhibits unique localization and cycling patterns.
- These features suggest a role for the Golgi complex in autophagy.
Conclusions:
- Atg27's trafficking suggests the Golgi complex is involved in autophagosome formation.
- Comparing Atg27 and Atg9 trafficking provides insights into membrane sourcing for autophagy.
- Further research is needed to elucidate the precise function of Atg27 in the autophagy pathway.
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