Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

α4 Nicotinic Acetylcholine Receptor Signaling Exacerbates Type 2 Airway Inflammation.

Allergy·2026
Same author

Comparative immunogenic and structural analysis of virus-like particle and inactivated whole-virion vaccines against enterovirus D68.

Molecular therapy. Nucleic acids·2026
Same author

Functional analysis of genes enriched in male reproductive organs: validation via fertility assessment of 14 knockout mouse lines.

Frontiers in cell and developmental biology·2026
Same author

A periplasmic layer formed by an outer membrane lipoprotein governs the cell-envelope integrity and stiffness of Leptospira interrogans.

Communications biology·2026
Same author

Cryo-EM structures of a MexB-MexY chimeric efflux pump reveal that large open clefts are intrinsic to the MexY porter domain.

Acta crystallographica. Section F, Structural biology communications·2026
Same author

Structural and dynamic insights into the biased signaling mechanism of the human kappa opioid receptor.

Nature communications·2025

Related Experiment Video

Updated: Jul 17, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

Comprehensive analysis of gene expression in testes producing haploid germ cells using DNA microarray analysis.

Akiko Ike1, Keizo Tokuhiro, Mika Hirose

  • 1Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.

International Journal of Andrology
|February 15, 2007
PubMed
Summary

This study reveals significant testicular gene expression changes during germ cell differentiation. Key genes involved in sperm development were identified, offering insights into male fertility and reproductive processes.

More Related Videos

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
10:30

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

Published on: January 20, 2014

Related Experiment Videos

Last Updated: Jul 17, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
10:30

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

Published on: January 20, 2014

Area of Science:

  • Reproductive Biology
  • Molecular Genetics
  • Developmental Biology

Background:

  • Understanding testicular gene expression is crucial for comprehending male germ cell differentiation and its impact on fertility.
  • Haploid germ cell differentiation involves complex molecular events that are not fully elucidated.

Purpose of the Study:

  • To comprehensively analyze differential testicular gene expression patterns before and after haploid germ cell differentiation.
  • To identify novel genes and pathways involved in spermiogenesis using microarray analysis.

Main Methods:

  • Microarray analysis was performed on mRNA from adult and juvenile mouse testes (17 days postpartum).
  • Approximately 14,000 expressed sequence tag (EST) clones from the Mouse FANTOM Array ver.1 were utilized.
  • Statistical analysis (p < 0.05) identified genes with reproducible expression changes.

Main Results:

  • Out of 1315 differentially expressed genes, 46% showed a twofold or greater increase in adults, and 22% showed a twofold or greater decrease.
  • Confirmed haploid-specific expression of known genes and identified novel upregulated genes (e.g., Allc, Skd3) and downregulated genes (e.g., hbb b1).
  • Genes were functionally classified into groups, including those encoding enzymes, structural proteins, chaperones, haemoglobins, and ribosomal proteins.

Conclusions:

  • Significant shifts in testicular gene expression occur during haploid germ cell differentiation, impacting various cellular functions.
  • The identified differentially expressed genes provide new molecular targets for understanding male reproductive health and infertility.