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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 17, 2026

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Identifying new human oocyte marker genes: a microarray approach.

Stéphan Gasca¹, Franck Pellestor, Saïd Assou

¹CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, Montpellier, F-34295, France.

Reproductive Biomedicine Online

|February 15, 2007

Summary

Identifying new gene expression markers, like BARD1 and BUB1B, can improve embryo selection in in vitro fertilization (IVF). This enhances the potential for successful pregnancy by assessing oocyte and embryo competence more effectively.

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Last Updated: Jul 17, 2026

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Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

Area of Science:

Reproductive Biology
Genomics
Assisted Reproductive Technology

Background:

In vitro fertilization (IVF) success is limited by poor implantation and pregnancy rates.
Current embryo selection relies on subjective criteria, lacking reliable markers for developmental potential.
Gene expression analysis offers a promising avenue for objective assessment of oocyte and embryo competence.

Purpose of the Study:

To identify novel gene expression markers for assessing human oocyte and embryo competence.
To explore the utility of microarray technology for comprehensive gene profiling in reproductive cells.
To discover new potential regulators and biomarkers for successful pregnancy outcomes.

Main Methods:

Microarray analysis of approximately 30,000 genes using U133P Affymetrix gene chips.
Profiling gene expression in human oocytes and cumulus cells.
Utilizing reverse transcription-polymerase chain reaction for targeted gene validation (implied).

Main Results:

Established gene expression profiles for a wide range of genes in human oocytes and cumulus cells.
Identified novel potential marker genes, including BARD1, RBL2, RBBP7, BUB3, and BUB1B.
These identified genes are involved in critical processes such as oocyte maturation.

Conclusions:

Gene expression profiling via microarrays can identify novel biomarkers for embryo selection in IVF.
New markers like BARD1, RBL2, RBBP7, BUB3, and BUB1B show potential for improving assessment of oocyte and embryo competence.
This approach may lead to enhanced IVF success rates by enabling better selection of embryos with higher developmental potential.