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Related Experiment Video

Updated: Jul 17, 2026

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture
06:16

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

Published on: August 10, 2019

Mice cloned from skin cells.

Jinsong Li1, Valentina Greco, Géraldine Guasch

  • 1Laboratory of Developmental Biology and Neurogenetics, The Rockefeller University, New York, NY 10021, USA.

Proceedings of the National Academy of Sciences of the United States of America
|February 15, 2007
PubMed
Summary

Adult skin stem cells, specifically keratinocyte stem cells (KSCs), are effective donors for cloning by nuclear transfer (NT). These readily accessible cells can be reprogrammed, offering a promising source for regenerative medicine and cloning applications.

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Area of Science:

  • Stem Cell Biology
  • Reproductive Biology
  • Dermatology

Background:

  • Adult stem cells possess self-renewal and undifferentiated properties.
  • Quiescent stem cells are hypothesized to be efficient donors for nuclear transfer (NT).
  • Previous research has not extensively tested skin-derived stem cells for NT.

Purpose of the Study:

  • To investigate the efficacy of adult skin stem cells as nuclear transfer donors.
  • To compare the efficiency of keratinocyte stem cells (KSCs) versus transit-amplifying (TA) keratinocytes for cloning.
  • To determine if sex influences cloning success rates using skin-derived stem cells.

Main Methods:

  • Isolation and use of nuclei from adult hair follicle stem cells and other skin keratinocytes for NT.

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Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

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Related Experiment Videos

Last Updated: Jul 17, 2026

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture
06:16

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

Published on: August 10, 2019

Generation of Mice Derived from Induced Pluripotent Stem Cells
11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
06:51

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Published on: April 29, 2016

  • Generation of cloned mice using KSCs, embryonic keratinocytes, and cumulus cells as nuclear donors.
  • Comparison of cloning efficiencies and survival rates between different cell types and sexes.

    • Main Results:

    • Liveborn cloned mice were produced using KSCs, with 9 surviving to adulthood.
    • Cloning efficiencies were similar across cell types (<6% per transferred blastocyst).
    • Adult KSCs demonstrated higher NT donor efficiency than TA keratinocytes, particularly in males.
    • Cloning success rates were consistently higher for male than for female recipients.

    Conclusions:

    • Adult skin stem cells, including KSCs, are viable and accessible donors for nuclear transfer.
    • Reprogramming of skin cell nuclei to pluripotency is achievable via oocyte cytoplasm.
    • These findings highlight the potential of skin as a source for stem cells in cloning and regenerative medicine.