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Updated: Jul 17, 2026
Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus (MRSA)
Published on: February 9, 2011
Characteristics of spore germination in a mouse model of cutaneous anthrax
Timothy S Bischof1, Beth L Hahn, Peter G Sohnle
1Division of Infectious Diseases, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, 53295, USA.
Background:
Cutaneous infection is the most common form of human anthrax, but little is known about Bacillus anthracis spore germination in these infections.
Methods:
We used experimental inoculations of B. anthracis Sterne spores or vegetative bacilli onto intact or abraded mouse flank skin, followed by evaluation of the infections and enumeration of germinating spores and vegetative bacilli.
Results:
Bacilli developed from a spore inoculum after application onto abraded, but not intact, skin of the mice. Germination appeared to occur extracellularly at the skin surface before the development of a phagocytic response; in fact, vegetative bacilli were seen after inoculation of the spores on top of a filter that separated them from the host phagocytic cells below. Malachite green staining demonstrated that spores began germinating 1-3 h after inoculation onto abraded skin. Vegetative bacilli were found not to be capable of initiating infection in the absence of cutaneous abrasion.
Conclusions:
The results indicate that epidermal damage is required for germination of B. anthracis spores in these infections; even so, spore germination by itself is not sufficient to produce infection of undamaged skin. In contrast to events in experimental inhalational anthrax, spore germination in these cutaneous infections appears to occur extracellularly.
