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Updated: Jul 17, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
First application of a microsphere-based immunoassay to the detection of genetically modified organisms (GMOs):
Anna Fantozzi1, Monica Ermolli, Massimiliano Marini
1Biotechnology and GMOs Unit, Institute for Health and Consumer Protection, DG-Joint Research Centre, European Commission, I-2120 Ispra (Va), Italy.
Abstract:
An innovative covalent microsphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for the quantification of the endotoxin Cry1Ab present in MON810 and Bt11 genetically modified (GM) maize lines. In particular, a specific protocol was developed to assess the presence of Cry1Ab in a very broad range of GM maize concentrations, from 0.1 to 100% [weight of genetically modified organism (GMO)/weight]. Test linearity was achieved in the range of values from 0.1 to 3%, whereas fluorescence signal increased following a nonlinear model, reaching a plateau at 25%. The limits of detection and quantification were equal to 0.018 and 0.054%, respectively. The present study describes the first application of quantitative high-throughput immunoassays in GMO analysis.
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