Fish oil ameliorates the allograft arteriosclerosis of intestine on rats

Fish oil had been shown to have an immunomodulating effect and had favorable effect on the pathogenesis of atherosclerosis. The aim of this study was to estimate the effect of fish oil on the graft arteriosclerosis and graft immune response after rat allogenic small intestinal transplantation. Since two wk pretransplantation, the Lewis rats were supplemented by gavage with: (i) phosphate buffer saline, 0.6% volume of body weight (V/W), as control group; (ii) fish oil (0.6%, V/W); (iii) fish oil (0.3%, V/W). Total intestine from donor F344 was heterotopically transplanted into the Lewis recipient. The recipient rats were killed on the 60th day post-transplantation. The cytokine levels, the activity of NF-kappa B and the arteriosclerosis of grafts were analyzed. Intragastrical supplementation with 0.6% fish oil induced pronounced inhibition of the pro-inflammtory cytokine expression of interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha, and the increase of anti-inflammtory cytokine expression of IL-10 in graft. Suppression of the TNF-alpha and IL-6 expression by fish oil was attributed to its inhibitory effect on NF-kappaB activation. Intragastric 0.6% fish oil infusion ameliorated the development of graft arteriosclerosis. Fish oil was therefore considered to have an immunosuppressive effect on rat allogenic small intestinal transplant model based on the intra-graft IL-6, TNF-alpha and IL-10 levels. It might result in ameliorating the arteriosclerosis of the grafts.