Unique use of alternative polyadenylation signals in the mouse aldolase B gene

We isolated and sequenced two mouse aldolase B cDNAs. They differ only in the length of the 3' untranslated region. This is consistent with Northern blot analysis of liver RNA which shows two transcripts differing by 400 nucleotides. We also isolated and sequenced the corresponding 3' genomic region and found four polyadenylation signals in the final exon. RNase protection studies demonstrate that all four of these signals are utilized, but not equally. This is unique to the mouse aldolase B gene.