Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NAXD Deficiency: Heterogeneous Phenotypes and Positive Response to Niacin Treatment.

Journal of inherited metabolic disease·2026
Same author

Somatic mosaicism in hypothalamic hamartoma.

Current opinion in genetics & development·2026
Same author

Genome sequencing boosts diagnostic yield for the developmental and epileptic encephalopathies.

medRxiv : the preprint server for health sciences·2026
Same author

Systematic analysis of snRNA genes reveals frequent RNU2-2 variants in dominant and recessive developmental and epileptic encephalopathies.

Nature genetics·2026
Same author

Activating Ras-MAPK pathway variants drive hippocampal clonal competition in human epilepsy.

bioRxiv : the preprint server for biology·2026
Same author

Rare heterozygous de novo variants in RAPGEF2 are associated with a neurodevelopmental disorder.

Genetics in medicine : official journal of the American College of Medical Genetics·2026

Related Experiment Video

Updated: Jul 17, 2026

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
14:23

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Published on: March 7, 2014

Gene expression profiling analysis of the inner ear.

Michael S Hildebrand1, Michelle G de Silva, Tuomas Klockars

  • 1Department of Otolaryngology - Head and Neck Surgery, University of Iowa, Iowa City, IA 52242, USA. michael-hildebrand@uiowa.edu

Hearing Research
|February 16, 2007
PubMed
Summary

Gene expression profiling for inner ear research faces limitations. A new approach using gene ontology and tissue enrichment data improves the discovery of genes crucial for auditory and vestibular function.

More Related Videos

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection
09:18

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection

Published on: March 8, 2017

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
22:02

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation

Published on: June 30, 2012

Related Experiment Videos

Last Updated: Jul 17, 2026

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
14:23

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome

Published on: March 7, 2014

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection
09:18

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection

Published on: March 8, 2017

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
22:02

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation

Published on: June 30, 2012

Area of Science:

  • Molecular Genetics
  • Otolaryngology
  • Genomics

Background:

  • Gene expression profiling has identified numerous genes related to inner ear function.
  • Current methods often miss low-abundance transcripts and genes with compensated functions.
  • This limits the discovery of genes critical for auditory and vestibular systems.

Purpose of the Study:

  • To review the limitations of current gene expression profiling strategies for inner ear gene discovery.
  • To propose a more effective approach for identifying genes involved in cochleo-vestibular development and function.

Main Methods:

  • Analysis of limitations in current gene expression profiling techniques.
  • Proposal of a novel strategy combining hierarchical clustering by gene ontology with tissue enrichment data.

Main Results:

  • Current methods may overlook important low-abundance transcripts.
  • Some highly expressed genes may not be essential for hearing or balance due to functional compensation.
  • The proposed method enhances the discovery of relevant inner ear genes.

Conclusions:

  • Traditional gene expression profiling has inherent limitations for inner ear gene discovery.
  • A refined approach integrating gene ontology and tissue enrichment data offers a more effective strategy.
  • This enhanced method aids in identifying and functionally characterizing genes vital for the cochleo-vestibular system.