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Updated: Jul 16, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
Role for actin in the polarized release of rotavirus
Agnès Gardet1, Michelyne Breton, Germain Trugnan
1UMRS 538, Membrane Traffic and Signalization in Epithelial Cells, CHU Saint Antoine, 27 rue de Chaligny, 75012 Paris, France.
Abstract:
Rotaviruses are characterized by polarized release from the apical side of infected enterocytes, and the rotavirus VP4 spike protein specifically binds to the actin network at the apical pole of differentiated enterocytic cells. To determine the functional consequences of this VP4-actin interaction, fluorescence recovery after photobleaching experiments were carried out to measure the diffusional mobility of VP4 associated with the microfilaments. Results show that VP4 binds to barbed ends of microfilaments by using actin treadmilling. Actin treadmilling inhibition results in the loss of rotavirus apical preferential release, suggesting a major role for actin in polarized rotavirus release.
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