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Updated: Jul 16, 2026

Xenopus laevis as a Model to Identify Translation Impairment
Published on: September 27, 2015
Xenopus Xp54 and human RCK/p54 helicases functionally replace yeast Dhh1p in brome mosaic virus RNA replication
Isabel Alves-Rodrigues1, Antonio Mas, Juana Díez
1Departamento Ciencias Experimentales y de la Salud, Universitat Pompeu Fabra, Dr. Aiguader 80, 08003 Barcelona, Spain.
Abstract:
By using a Brome mosaic virus (BMV)-Saccharomyces cerevisiae system, we previously showed that the cellular Lsm1p-7p/Pat1p/Dhh1p decapping-activator complex functions in BMV RNA translation and replication. As a first approach in investigating whether the corresponding human homologues play a similar role, we expressed human Lsm1p (hLsm1p) and RCK/p54 in yeast. Expression of RCK/p54 but not hLsm1p restored the defect in BMV RNA translation and replication observed in the dhh1Delta and lsm1Delta strains, respectively. This functional conservation, together with the common replication strategies of positive-stranded RNA viruses, suggests that RCK/p54 may also play a role in the replication of positive-stranded RNA viruses that infect humans.
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